Most of hte sites I went through recommended 2 g/day, taken just before sleeping. I read somewhere that the best bet was to take 1g and hour before sleeping and another gram 2 hours after u've slept (waking you up with an alarm clock)! That's because the pituitary gland releases HGH just after falling asleep. So if you time your gaba dosage in that interval the blood level of HGH will add up! But then, I'm not that mad to wake up after 2 hrs!!