PGE-1 Storage Questions

goldmember said:
Well, PGE-1 is not a peptide, although you could be right about diluting actetic acid, it might get fairly close to pH7 (about 6.8 or so). Would you suggest reconstituting the IGF-1 and PGE-1 together?
Click to expand...
That's what i wanted to do... but if that won't work then i'll re-constitute them separately and draw in the same syringe just prior to injecting.
 
sikdogg said:
That's what i wanted to do... but if that won't work then i'll re-constitute them separately and draw in the same syringe just prior to injecting.
Click to expand...
That seems like the best thing to do.
 
if you had a 5mg vial of PGE-1, then would you reconstitute it all at the same time? with acetetic acid? how long would this last, say in the fridge?

also IGF-1 (which i would also use in IC injections), assuming you bought it in powder form, what would you use to reconstitute it? also ive read that IGF-1 doesnt last that long once mixed, so would you have to mix small amounts every day?

if you could reconstitute them both and draw into the same syringe, and both have a long shelf life - then that would be the ideal situation id look for. any ideas?
 
I would re-constitute them separately... IGF-1 in an acetic acid solution and PGE in Bacteriostatic Water. Draw them both up in the same syringe and inject. This allows you better control of dosages and the ability to increase or decrease one or both of the compounds.

IGF-1 can last for months if refridgerated and in an acetic acid solution. PGE-1 will last a few months if refridgerated. Both will begin to degrade soon after re-constitution but the degradation process is slow. Even if they degrade to 50% after a few months, there's still enough active ingredients to make it work...
 
ok thanks sikdogg. a few months supply of igf and pge1 wont actually be that expensive. so will prob start this soon... just have to get over the fear of needles!! anyone know where i can buy an auto-injector from?
 
damn edit button >:(

any ideas on what concentrations to use? i would plan on doing about 20mcg of IGF, and then titrating the PGE-1. so do you think 50 mcg/ml? i might buy the PGE-1 by the mg - to give more room for mixing...

also, with acetic acid - how would you go about mixing it? surely you would need to test the pH, but what would you use to accurately measure the pH?
 
EVO said:
ok thanks sikdogg. a few months supply of igf and pge1 wont actually be that expensive. so will prob start this soon... just have to get over the fear of needles!! anyone know where i can buy an auto-injector from?
Click to expand...
For IC injections, you will need to use insulin syringes... you can easily buy an auto injectors like Inject-ease. You place the loaded syringe in the unit and it will automatically do the initial poke, you just have to do the actual pushing of the plunger to inject. Trust me... it makes self-injecting a breeze.
 
EVO said:
damn edit button >:(

any ideas on what concentrations to use? i would plan on doing about 20mcg of IGF, and then titrating the PGE-1. so do you think 50 mcg/ml? i might buy the PGE-1 by the mg - to give more room for mixing...

also, with acetic acid - how would you go about mixing it? surely you would need to test the pH, but what would you use to accurately measure the pH?
Click to expand...
I would re-constitute at a concentration that is easy to work with to start... i would do it at 100mcg/ml. This makes dosing on a U-100 sytinge a no-brainer. If you want to inject 10mcg, you simply draw up .1ml

I can post an easy way of diluting and sterile filtering acetic acid for IGF if you're interested...
 
So the increments on u-100 syringes are .1ml at the smallest? Increments of 2.5mcg are necessary for PGE-1 titration, so in that case it seems like it would be better to dilute the PGE-1 to 25mcg/ml. With 100mcg/ml, the little bit of PGE-1 left in the syringe would make a big difference in erection time.

I remember caverject being something like 20mcg/ml.
 
spinner2 said:
So the increments on u-100 syringes are .1ml at the smallest? Increments of 2.5mcg are necessary for PGE-1 titration, so in that case it seems like it would be better to dilute the PGE-1 to 25mcg/ml. With 100mcg/ml, the little bit of PGE-1 left in the syringe would make a big difference in erection time.
Click to expand...
Some 1cc u-100 syringes have .1cc divisions or tick marks but i think most have .2cc tick marks. I prefer using .3cc syringes as i can gte them with thinner needles (31g) and they have .1cc divisioons.

I remember caverject being something like 20mcg/ml.
Click to expand...
It doesn't really matter what concentration you re-constitute at... it's the dosing that matters. The difference in concentration changes the volume you have to inject to get to a certain dose, nothing more.
 
EVO said:
that would be great if you could!!
thanks!
Click to expand...
Here you go....

Originally Posted by RedBaron
Reconstituting IGF-1 with white vinegar
I have noticed several posts lately wondering about a good method for reconstituting IGF-1 and a few posts about using vinegar/water to do so. I originally wrote this post at anabolicmonsterz, but since there seems to be a reasonable interest here as well, I will post it for those that might be interested.

I began using this method many months ago ... mainly out of impatience. I was out of Benzyl Alcohol, and while I had access to HCl, it wasn't accessible when I wanted to begin my Long R3 IGF-1 cycle. I decided to use plain ol' grocery store Distilled White Vinegar. I used the following, which has worked out really well for me....and the feedback I have had from others that I have shared it with has been positive so far. So here it is for anyone that is interested:

The calculation:
Distilled white vinegar is supposed to be standardized to ~5% acetic acid, which would make it 850mM. To get it to the recommended 100mM, you'd want 11.76% white vinegar (100mM/850mM = 11.76%). Since it would be almost impossible to draw out 11.76IU's, I round this to 12, which is certainly going to be close to our desired 100mM.

The filtering process:
I use off the shelf grocery store distilled white vinegar. In order to ensure safety, I filter it using .20u whatman filters. Here is the step by step for those that may not be familiar with filtering using whatmans. What you will want to have on hand before starting out is some sterile vials, some .20u whatman filters, some syringes and needles (I use a 10cc syringe, and .23 gauge 1" needles), and some alcohol swabs.

(1) First draw up about 10cc of the distilled white vinegar
(2) screw on the .20u whatman to the 10cc syringe (or whatever size you use)
(3) screw on a .23 gauge needle (or whatever size you decide to use)
(4) take your sterile vial, swab the top with alcohol, insert a needle for venting.
(5) Insert your syringe/whatman/needle apparatus and slowly push the 10cc's into the sterile vial.

Now you have safe vinegar to use for your reconstituting.

ALTERNATE METHOD - Alternately, you could simply mix your water and distilled white vinegar before filtering using about 7.5 parts of water per 1 part of distilled white vinegar. After mixing these together in this ratio, run the mixture through your .20u whatman as above. You will end up with a vial of dilutent this way that has the proper PH for use with your IGF-1.

Reconstituting:

How much water/vinegar you reconstitute with is going to somewhat depend on which LR3 IGF-1 you are using. Igtropin is shipped in 100mcg vials, which I usually reconstitute at 1ml(cc) per 100mcg vial (which will make the 10 mark on your insulin syringe = 10 mcgs). The gropep based IGF-1's are primarily shipped in 1mg vials, and I usually use 5ml for these (which will make the 10 mark on your insulin syringe = 20mcgs).
At any rate, what I do is:

(1) take an alcohol swab and swab the tops of my water, vinegar solution, and IGF-1 vials
(2) take a syringe with a 23 gauge, 1" needle and draw out .12 cc's of vinegar for the 100mcg vials or .60 cc's for the 1mg vials.
(3) next I take this syringe and draw out the water - .88cc's for 100mcg, 4.4cc's for the 1mg.

FOR ALTERNATE METHOD in lieu of steps (2) and (3) - Just draw out the desired amount of dilutent from your pre-mixed vial of
vinegar / water.

(4) next i poke the needle into the LR3 IGF-1 vial and dribble this solution down the side of the vial, avoid any direct spray on the lyophilized powder until all of the dilutent is in the vial
(5) using a gentle swirling motion, I reconstitute the powder.
(6) I stick the vial in the fridge and it is now ready for use.

Well, I think that about sums it up. Hope this helps anyone who may have been wondering about using vinegar to reconstitute. I would advise that if you end up using Igtropin, you seriously consider using this vinegar method. Igtropin and other dilutents such as BA do not get along well together at all.

RedBaron
Click to expand...
 
I do it all the time... in fact, i just placed an order for more. I will also be tryiing out a new automatic injector...

Autoject 2
 
does anyone know if taking pain killers like ibuprofen will affect the efficiency of PGE-1 treatment? i know it affects prostaglandins, but does it affect PGE-1? cheers if anyone knows
 
EVO said:
does anyone know if taking pain killers like ibuprofen will affect the efficiency of PGE-1 treatment? i know it affects prostaglandins, but does it affect PGE-1? cheers if anyone knows
Click to expand...
Anti-inflammatories block the formation of PG's by inhibiting the COX enzyme. since you are injecting the PGE-1, the NSAIDs should have no effect on it.
 
thanks gold. wasnt sure if they affected the synthesis or degradation. thanks
 
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