PGE-1 Storage Questions

spinner2 · Apr 4, 2007

Alright, I've got 5mg of PGE-1. I'm storing it in a vial in my freezer right now, as was directed in the chemical instructions.

I've been reading about reconstituting it online, and websites say I should inject within 24 hours of reconstituting it. Why is this?

Since I have no possibility of measuring 20mcg increments, which is what I will probably inject, what would you suggest I do?

spinner2 · Apr 4, 2007

I found one study saying that in solution of pH ~ 7 that the pge-1 degraded to 76% concentration after 60 days. Not sure about what temperature the study was at, but storage in a freezer slows the degradation slightly. I also know that the degradation follows a logaritHydromaxic curve with r=0.99+ and that the solution will stay stable at over 3 months from another study.

I'll probably mix 1mg at a time in 12.5ml bacteriostatic water. Then plot the logaritHydromaxic curve, to modify my doseage over time. It'll be a little trial and error, but the minor doseage changes should be ok.

Hopefully this works out well

spinner2 · Apr 4, 2007

Yes, I found another study saying that the concentration is 90% after 82 days at 4 degrees celsius! And 50% after 540 days. This means I should be able to mix 1 mg at a time and leave it in aqueous solution with only 1 doseage increment when my erections go down under 2 hours.

This makes everything so much easier for me

.

stjohncock · Apr 4, 2007

I have a bottle of quadmix from Boston Medical that has PGE1 as one of its constituents. The instructions say to keep it refrigerated but not to freeze. This is, of course, already reconstituted. The expiration date on the bottle is 6 months.

spinner2 · Apr 4, 2007

stjohncock said:
I have a bottle of quadmix from Boston Medical that has PGE1 as one of its constituents. The instructions say to keep it refrigerated but not to freeze. This is, of course, already reconstituted. The expiration date on the bottle is 6 months.

Thanks for the reply. Chemical Penis Enlargement set to officially kick off as soon as I get a little more money for other ingredients.

sikdogg · Apr 4, 2007

Did any of the studies you read tell at what ph the PGE will remain stable in?? If you know this, then it's a simple matter of re-constituting it in the correct ph solution to keep it stable. This is what is done to kep IGF stable.

spinner2 · Apr 5, 2007

Yes. Stability is best at pH 7. Studies have demonstrated stability at a wide range, though. I remember 10.4 as the upper bound, and somewhere around 3 as the lower bound.

spinner2 · Apr 5, 2007

Another interesting thing I found was that PGE-1 degraded much more quickly in the presence of papavarine, which is a common trimix ingredient.

EVO · Apr 5, 2007

wow - very interesting. this was a problem i thought of if i bought a 5mg supply of PGE - 1. seems more sense to buy it like this since caverject you are wasting so much!! keep us posted on how you get on!
btw - what else are you going to mix in with it? igf?

dogsdoodahs · Apr 5, 2007

I got quadmix from BathmateG and I asked if I could freeze some.
They instructed me not to freeze it but to keep it at 4-6 degrees and it should be good for 6 months.

it contains
papaverine, clorapromazine, atropine, alprostadil

dogsdoodahs · Apr 5, 2007

I want to do an experiment because the amount of quadmix i have is going to last me well over 6 months and past the expiry date.

Even though BathmateG told me not to freeze it i want to see if when i use it what difference it makes.

I am going to freeze about 3ml. I have an small empty bottle. Will pouring in boiling water and emptying it out again be sufficient to sterilize it?
Can I then just pour in the quadmix and freeze it without worry of infecting myself when i thaw it out and inject it over the following few months?

EVO · Apr 5, 2007

boiling water is not sufficient enough to kill all pathogens. you could buy steralisation tablets?

sikdogg · Apr 5, 2007

spinner2 said:
Yes. Stability is best at pH 7. Studies have demonstrated stability at a wide range, though. I remember 10.4 as the upper bound, and somewhere around 3 as the lower bound.

Makes sense since the body's PH level is right around 7.5. That said, re-constituting it in an acetic acid solution like IGF-1 should keep it stable for extended periods of time.

goldmember · Apr 5, 2007

By freezing it, what method will you use to thaw it when it is time to inject?

SD,

Why would you say that acidic solution (acetic) would keep it stable for long periods of time? Why not pH 7.5?

spinner2 · Apr 5, 2007

I have the 5mg of PGE-1 in my freezer, not solution. When I reconstitute it I'll just store it in my refrigerator, because it would be easier to deal with and would last long enough.

goldmember · Apr 5, 2007

spinner2 said:
I have the 5mg of PGE-1 in my freezer, not solution. When I reconstitute it I'll just store it in my refrigerator, because it would be easier to deal with and would last long enough.

Even the raw powder is perishable at room temperature? It would be wise to consider adding dessicant to the container of PGE-1 powder (not mixed, but in the same container, careful to keep separate). A vacuum seal would also be a good idea.

sikdogg · Apr 5, 2007

goldmember said:
... Why would you say that acidic solution (acetic) would keep it stable for long periods of time? Why not pH 7.5?

I could be wrong but wouldn't diluting acetic acid to 100mM get the ph close to 7?? This is what's used for igf and since they are both human peptides, they should both require the same ph level.

spinner2 · Apr 6, 2007

goldmember said:
Even the raw powder is perishable at room temperature? It would be wise to consider adding dessicant to the container of PGE-1 powder (not mixed, but in the same container, careful to keep separate). A vacuum seal would also be a good idea.

It was carefully packed and sealed already. Well enough to be stable at least 2 years, according to the papers I got.

goldmember · Apr 6, 2007

sikdogg said:
I could be wrong but wouldn't diluting acetic acid to 100mM get the ph close to 7?? This is what's used for igf and since they are both human peptides, they should both require the same ph level.

Well, PGE-1 is not a peptide, although you could be right about diluting actetic acid, it might get fairly close to pH7 (about 6.8 or so). Would you suggest reconstituting the IGF-1 and PGE-1 together?

dogsdoodahs · Apr 6, 2007

EVO said:
boiling water is not sufficient enough to kill all pathogens. you could buy steralisation tablets?

in that casei am pleased i asked, cheers evo.
i'll scrap that idea.

PGE-1 Storage Questions

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